Insertion junction: CLIP2.048876_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048876
Chromosome:chromosome 1
Location:7672559
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053850 (1 of 2) K10143 - E3 ubiquitin-protein ligase RFWD2 (RFWD2, COP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGAAGATGCGTGCAGCTGCAGCGCTGCCCGGCCGTTGTGGCCCTCGCA
Internal bar code:CATGGTAGCATCGGATGGGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4874
LEAP-Seq percent confirming:98.9474
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTGCCTGTTCTGTCAGT
Suggested primer 2:CGTCATCGTCCTCCAGATCG