Insertion junction: CLIP2.048898_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048898
Chromosome:chromosome 16
Location:4188916
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670250 TOM6 (1 of 1) K14964 - Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2); Translocase of mitochondrial outer membrane CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTCCCGACGCCGCCGCCGCCAGCTCGCACACGGGCGTGGGCGGCGGGC
Internal bar code:TCTTTATTATTGCTCCACTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:994
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAAATCAACCTGGTGCGC
Suggested primer 2:CAAACAGCAACCCGTTCCAG