| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049019 |
| Chromosome: | chromosome 2 |
| Location: | 3952844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102350 | ATG3,APG3 | Autophagy-related protein; (1 of 1) K08343 - ubiquitin-like-conjugating enzyme ATG3 (ATG3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTTGGGGGGAAAGTACGTGCGGCGCTTCTTGGGGTCGCCGCCCTCC |
| Internal bar code: | ATATTGTAAAGACTTTAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3795 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTACGATGCTGGCTCAT |
| Suggested primer 2: | CAAACTCGCTGTTGGGGTTG |