| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049983 |
| Chromosome: | chromosome 6 |
| Location: | 3005662 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274350 | HTR5,HTR29 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTACCAGAAGTCGACTGAGCTGCTGATCCGCAAGCTGCCATTCCAGC |
| Internal bar code: | CGTTCGGCGCTCGGCTTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2645 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGTGGACGCATCAGGA |
| Suggested primer 2: | CTTGGTGATGCCCTGGATGT |