Insertion junction: CLIP2.050027_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050027
Chromosome:chromosome 5
Location:708828
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g245351 (1 of 1) PF00059//PF00530//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGAGAGATGGAGGGGCCCTCGTATCGGCTGTCTGTGCCGCCGCCCACG
Internal bar code:AAAGCCCAGTAGGCGTCTAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:555
LEAP-Seq percent confirming:65.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCTTGAAGACCCCTCGT
Suggested primer 2:CTGCCCCGCATTTCACAAAA