| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050704 |
| Chromosome: | chromosome 12 |
| Location: | 3665789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513500 | XAB2 | (1 of 1) K12867 - pre-mRNA-splicing factor SYF1 (SYF1, XAB2); Transcription-coupled DNA repair protein, possible splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATTGTATAATTGTGCCTGCCTGCACAGTTACTGGTGATTAGCCCGGG |
| Internal bar code: | CGGAGAACGTCTCATGCGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTCTTGCAATGCCACGC |
| Suggested primer 2: | CTCTTATGCTGGAAACGCGC |