Insertion junction: CLIP2.050704_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050704
Chromosome:chromosome 12
Location:3665797
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g513500 XAB2 (1 of 1) K12867 - pre-mRNA-splicing factor SYF1 (SYF1, XAB2); Transcription-coupled DNA repair protein, possible splicing factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAATACACAGGATTACAGAGAAGCAGCACAGCCTAGCAGGACGACTCAC
Internal bar code:TACGAGTTTCTTCACTGGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2760
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTATGCTGGAAACGCGC
Suggested primer 2:TTCTTCTTGCAATGCCACGC