| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050745 |
| Chromosome: | chromosome 2 |
| Location: | 1450644 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083700 | LSP1 | Signal transducer for phototaxis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGTGTCACCCCATGTTAGGCCGCTTCTGGGGCCTTCACCTGCCCCA |
| Internal bar code: | CGTGATCTTTTTTTTGAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1716 |
| LEAP-Seq percent confirming: | 32.3077 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCTTTGTTCCTCGTCA |
| Suggested primer 2: | TGACTTCGCTTTGCAAGTGC |