| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050987 |
| Chromosome: | chromosome 3 |
| Location: | 8074403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201300 | SSA10 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR10157//PTHR10157:SF27 - DOPAMINE BETA HYDROXYLASE RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCAACTTGGCGCCCAGCCTCGCGCTGCACTGGAGTGTCAGCGGTGGCT |
| Internal bar code: | GGCCTCCCGACGCGCGCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4414 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGGGCTCAGGAATCAT |
| Suggested primer 2: | GGTGGAGCGTGTGAAGAAGA |