| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051014 |
| Chromosome: | chromosome 12 |
| Location: | 3145945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500600 | VMA21 | (1 of 1) PF09446 - VMA21-like domain (VMA21); vacuolar ATPase assembly factor | gene_edge/mRNA_edge/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGCATGCCACCTTCCGCGACGGCCGGAGGCAGCCTGCAGAGACCGCA |
| Internal bar code: | AACCAATTTGTTATGTGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3389 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCCGTAACCAACCAAC |
| Suggested primer 2: | AATTTTGTTGGCGGTGAGGC |