Insertion junction: CLIP2.051069_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051069
Chromosome:chromosome 13
Location:1186674
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569801 (1 of 2) K13172 - serine/arginine repetitive matrix protein 2 (SRRM2, SRM300) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGGGATGCAAGGCGGGCTGGCCTAAGTACACGTATTAAGTGCCGACAT
Internal bar code:TGGGCAGTATCCTGAATTGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1199
LEAP-Seq percent confirming:88.5714
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCCAATGTGCGTGTGGG
Suggested primer 2:CTCCCCCAAACTCCCATTCC