| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051400 |
| Chromosome: | chromosome 3 |
| Location: | 4067533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172100 | PDF1A | Peptide deformylase; (1 of 1) PTHR10458//PTHR10458:SF11 - PEPTIDE DEFORMYLASE // PEPTIDE DEFORMYLASE, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCCCCTGTCCCCGCTCCTGTGCCGGCCCGCACGCTGGTTGGGGTTG |
| Internal bar code: | AAGGACGATTGATATATCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2291 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCCCTCTCTTCCCTCAT |
| Suggested primer 2: | TGGCATCGAAAATCCACGGA |