| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051548 |
| Chromosome: | chromosome 12 |
| Location: | 5317661 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528400 | (1 of 1) PTHR22938:SF0 - ZINC FINGER PROTEIN 598 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGCACCTTCCGGCTCTTGACGCACACGTCACAGAAGAACTGGCCCGA |
| Internal bar code: | TCATCCCTGATGTAGAAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5585 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCACACCGTTGTTTCC |
| Suggested primer 2: | AACACAACGAACTTGCGCTC |