Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.051570 |
Chromosome: | chromosome 5 |
Location: | 2943678 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre24.g755347 | MUT6 | Transgene and transposon silencing 6; (1 of 1) K12815 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX38/PRP16 (DHX38, PRP16) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAACAGTCAAGGCAGGGGGTGGGGAGGAGGCAGGGAGAGTGTGGGT |
Internal bar code: | GGGGTTTCCGCATTGACATGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 207 |
LEAP-Seq percent confirming: | 76.9231 |
LEAP-Seq n confirming: | 10 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGGTTTGAGATCCTCGCAA |
Suggested primer 2: | CTTGCTCCTTGTTGTGCCAC |