| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051631 |
| Chromosome: | chromosome 14 |
| Location: | 491068 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611100 | FAP27 | Flagellar Associated Protein 27 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGTACCGGTGTTGGTAGTAGCGGTGGCAGTGGGAGTGAGGAGGCCA |
| Internal bar code: | GTCGCGATTCCTGGAACCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTGTGCGTGTGGGAGA |
| Suggested primer 2: | GTCGCCCAGGAATTCCTCAA |