| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051668 |
| Chromosome: | chromosome 16 |
| Location: | 1497175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652950 | (1 of 1) K11274 - chromosome transmission fidelity protein 4 (WDHD1, CTF4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACTGGTATCTACATCCCCCCTCCCCTCCATCCTCACCGCCGCCCGC |
| Internal bar code: | CCGTGAATTTATCTCTGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2473 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTAAGGCCGCTTAGGA |
| Suggested primer 2: | TGACACACACGGATCACCTG |