Insertion junction: CLIP2.052019_6

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052019
Chromosome:plastome
Location:92733
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802303 2717010,ChreCp039,rps9 30S ribosomal protein S9; (1 of 2) PTHR21569//PTHR21569:SF1 - RIBOSOMAL PROTEIN S9 // 28S RIBOSOMAL PROTEIN S9, MITOCHONDRIAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACCCATTACCTTCTTTAATTTGAACTTGAGCTACCGCTTCTTTACGAC
Internal bar code:TAGGGGGGAATAGAACAGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:21.0526
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGACAAGCATTTTCCCGA
Suggested primer 2:CCAAAGGACGGCAATGAACG