| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052181 |
| Chromosome: | chromosome 4 |
| Location: | 156597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216850 | TUA2 | (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGAAGAAGGTGTTGAAGGCATCGTCGCCACCGCCAATGGTCTTGTCG |
| Internal bar code: | GCGTCCGAGTGTGGGTCTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1702 |
| LEAP-Seq percent confirming: | 57.3529 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTCCCCGCTTCTATCC |
| Suggested primer 2: | GTGGACAGCACCGAGTTGTA |