Insertion junction: CLIP2.052511_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052511
Chromosome:chromosome 2
Location:4174135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g103950 MIA1,FAP100 (1 of 2) PF13863 - Domain of unknown function (DUF4200) (DUF4200); MIA1 subunit of the modifier of inner arms (MIA) complex intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACGGCATCACCCGCCACGAGAGGCTACCGCGTGCACGGGAGGCGCA
Internal bar code:TCCTTTGGTTTTAGTGTAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5606
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:112

Suggested primers for confirmation by PCR

Suggested primer 1:GGGACAAACGAACAAGCCAC
Suggested primer 2:GTACCACCACCTGTGTCCAG