| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.052558 |
| Chromosome: | chromosome 16 |
| Location: | 3972075 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689650 | ATG8,APG8 | Autophagy-related protein; (1 of 1) K08341 - GABA(A) receptor-associated protein (GABARAP, ATG8, LC3) | intron |
| Cre16.g689700 | HPR5 | (1 of 1) 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP(+)); Hydroxypyruvate reductase 5 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCTTGCCGCGCCTCGCTCACAGATAAGCGGAAAGCGGAGGCCGCAC |
| Internal bar code: | TTAGGGTGGATGAGAGGCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1455 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTTTATCCGCTTGC |
| Suggested primer 2: | AGCAAGTTCACTGAGGACCG |