| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053034 |
| Chromosome: | chromosome 12 |
| Location: | 9308655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542250 | TUB1,RSP19 | (1 of 2) K07375 - tubulin beta (TUBB); beta-1 tubulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCATGGATTCCGTGCGCTCGGGCCCCTACGGCCAGATTTTCCGCCCGG |
| Internal bar code: | GTCATATCCAGGGCATAAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5050 |
| LEAP-Seq percent confirming: | 94.9367 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCCTGAGGGAAGGGAAC |
| Suggested primer 2: | CCCAATTGCATCCTCAGGGT |