| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053086 |
| Chromosome: | chromosome 11 |
| Location: | 3883745 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479750 | SRP54,cpSRP54,SRP54L | Chloroplast SRP54; (1 of 1) PTHR11564:SF7 - SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCATACTGACCACGAAGGTGAGCAGATGCAACAGGTGCAACCGCAG |
| Internal bar code: | GCGTAAACATGAGGTGGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3351 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTAGGGACGTGTATGGT |
| Suggested primer 2: | GCAATAGATGGGCGTGCATG |