| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053554 |
| Chromosome: | chromosome 12 |
| Location: | 7666256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555200 | (1 of 1) PF09786 - Cytochrome B561, N terminal (CytochromB561_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTGTGCGGTGTCGCTCAAGGTAGTTCCTGTTTGGGCCGACATGCCC |
| Internal bar code: | GACCAGTAGCACCGGACCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1111 |
| LEAP-Seq percent confirming: | 34.0909 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACACTGTCAGCACGG |
| Suggested primer 2: | CAAGGCCTACAGTCACAGCA |