| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053666 |
| Chromosome: | chromosome 12 |
| Location: | 8335129 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550900 | SSD3 | Sterol sensing domain protein; (1 of 1) K12385 - Niemann-Pick C1 protein (NPC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTCCAGGTGGGCGGCACCTGCGACCTGTGGCTGCAGCGCTCTAGGGG |
| Internal bar code: | TCGTTGAGTGCTGAATGCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2248 |
| LEAP-Seq percent confirming: | 65.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTTGGGAATTCGGGGAC |
| Suggested primer 2: | GCGCCTCTCCACAGATCTAC |