| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053945 |
| Chromosome: | chromosome 12 |
| Location: | 4378428 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519500 | CAX1,VCX1 | (1 of 3) K07300 - Ca2+:H+ antiporter (chaA, CAX); CAX family cation antiporter, membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCATGTAACATACCAAAGTCGGCTGGCCGGTTTAAGACTTTGCCGG |
| Internal bar code: | ACTCGATTTCTTTTGAACCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3603 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGGTTTGCACACAAAG |
| Suggested primer 2: | ATTCCGTCGGGATGTGATCG |