| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053974 |
| Chromosome: | chromosome 3 |
| Location: | 4793169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177950 | (1 of 1) PTHR32093:SF10 - MUCIN 14A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTATCAACAAAAGCAGCGATCGGCAGTACGTCCGAGACACGAGGTCC |
| Internal bar code: | ACGGGCCAATACTATGAGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2126 |
| LEAP-Seq percent confirming: | 29.5775 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGAGCGAAATCTGCCTA |
| Suggested primer 2: | CCAGGCTTCCAGTACAAGCA |