| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054405 |
| Chromosome: | chromosome 12 |
| Location: | 7666065 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555200 | (1 of 1) PF09786 - Cytochrome B561, N terminal (CytochromB561_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGGCGTCTGCAGGGTGGAAGGGGGATAGCACAGTTAGCGCGGGCCA |
| Internal bar code: | GACGCTGCCCGATTTCGTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1946 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACACTGTCAGCACGG |
| Suggested primer 2: | CAAGGCCTACAGTCACAGCA |