| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054452 |
| Chromosome: | chromosome 12 |
| Location: | 5258037 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527750 | LC2,ODA12,DLT2,DLC2,ODA-LC2,Tctex2 | Flagellar Outer Arm Dynein Light Chain 2; (1 of 1) PTHR21255//PTHR21255:SF25 - T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCCCTTCACGGGGTCATATTTTTGCTTCTCAATGCGCTCCTTCAG |
| Internal bar code: | GTCTAAGATCTCTTTTTAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4421 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACGAAAGAAGGGACGTT |
| Suggested primer 2: | AGTTGTCGTTCGTCTGGTCC |