Insertion junction: CLIP2.054460_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.054460
Chromosome:chromosome 17
Location:3872795
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre17.g727600 (1 of 1) PF06650//PF12624//PF16908//PF16909 - SHR-binding domain of vacuolar-sorting associated protein 13 (SHR-BD) // N-terminal region of Chorein or VPS13 (Chorein_N) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Vacuolar-sorting-associated 13 protein C-terminal (VPS13_C) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACGCAGGAGTCCGAGGGCGACAGGTCACATGCCTTAAAACCCGGTC
Internal bar code:CGAGTAGCATTAAGCTCTGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:549
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCTCTCAAGCTCAACCG
Suggested primer 2:ACGTGGTGCATAACGGACTT