| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054475 |
| Chromosome: | chromosome 6 |
| Location: | 3358288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278086 | (1 of 1) PTHR11803//PTHR11803:SF17 - TRANSLATION INITIATION INHIBITOR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCGGCAGTGACCACAATCTCAACCTGTTGGCGTGGTGGGGCCACAT |
| Internal bar code: | CATAACTCAATATGCCGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1107 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACGTTCGCTTGTCGTA |
| Suggested primer 2: | TGCCACATCTGCTTGGACAT |