Insertion junction: CLIP2.054748_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.054748
Chromosome:chromosome 11
Location:775553
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre11.g467631 CGL90 Zinc carboxypeptidase; Conserved in green lineage; (1 of 1) 3.4.17.15 - Carboxypeptidase A2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAAGGCGAAGTTGTAGCCAGGTGGGGGAGGGGAGGGGATTCAAGGCAT
Internal bar code:AGGCGCTGTGGCGAAGTGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:142
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACTTGCGACAGCTCCAT
Suggested primer 2:TATGGAGGACAGCGGAAGGA