| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055034 |
| Chromosome: | chromosome 2 |
| Location: | 6195084 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120250 | CDPK7,STT7 | (1 of 3) PTHR11584:SF316 - SERINE/THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC; Chloroplast protein kinase required for state transitions | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCCGATGCGCAGGTCGGCGGCGGCGCCCAGGTCAATCAGCTTGATC |
| Internal bar code: | TTGTTGACCCGTAAAGGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACAACCTGGAGCCACTG |
| Suggested primer 2: | CTATCACGCCTCAGACACCC |