Insertion junction: CLIP2.055181_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055181
Chromosome:chromosome 11
Location:4278220
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482150 (1 of 100) IPR001810 - F-box domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCCATCGTGCTGGACCGCTGCGCGTGCACTCTCGCTGGCGCGCGCCG
Internal bar code:TTTTGCGAAGTCTAAGTAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCTGTTTGTGTGAGTTG
Suggested primer 2:CTGATCCGGCTGTAGCACTG