Insertion junction: CLIP2.055245_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055245
Chromosome:chromosome 1
Location:3809518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024850 RDP2 Putative rhodanese domain phosphatase; (1 of 3) K18065 - Cdc25 family phosphatase [EC:3.1.3.48 1.20.4.-] (CDC25) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTAAGAGGCGGCTTCACCAGCTTTGTGCGAACCTACAAGCACGAGGA
Internal bar code:TTCCAGCTGATCGCCGGGACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCAAGGAGATCGCAACG
Suggested primer 2:AAAGCTGAGCTGGATGGCAT