Insertion junction: CLIP2.055331_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055331
Chromosome:chromosome 5
Location:2465304
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g239000 (1 of 1) IPR006626//IPR011050//IPR011989//IPR012334 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Armadillo-like helical // Pectin lyase fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCTGCACGATCAAACACTCCAAAATAGTCCAGCAGGTCCTCCCAGGCC
Internal bar code:TTAAAAACATATGCATGTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCGCTCATTTCACCCCT
Suggested primer 2:TCAAGGCACAACACAGTGGT