Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.055361 |
Chromosome: | chromosome 1 |
Location: | 4041704 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g026700 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGGCACCTGCTCGCCCACGCAGCGACTTGCCTTAGCTCACTAGCT |
Internal bar code: | TGGGTCCCTGTAAATGGTAGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2303 |
LEAP-Seq percent confirming: | 18.5185 |
LEAP-Seq n confirming: | 5 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGAAGAGAAGCAGCGTGTC |
Suggested primer 2: | TCGACAGCAGAAGGACAACC |