| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055405 |
| Chromosome: | chromosome 2 |
| Location: | 4777231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108750 | (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGACGCACAGCACCCCGCTTCGAATTTCCAATTCCTTCGATGGAATG |
| Internal bar code: | CTAACGTATTCATTTGAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3419 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGAGATTGCTGCGTGA |
| Suggested primer 2: | ACCTTGCGGTCCATTTCGAT |