| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055443 |
| Chromosome: | chromosome 16 |
| Location: | 7557030 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683495 | REX1-S,TFB5,REX1S | (1 of 1) K10845 - TFIIH basal transcription factor complex TTD-A subunit (TTDA, GTF2H5, TFB5); TFB5 subunit of general Transcription Factor II H, involved in Nucleotide Excision Repair | CDS |
| Cre16.g683595 | CAX4 | (1 of 1) PTHR12266//PTHR12266:SF0 - NA+/CA2+ K+ INDEPENDENT EXCHANGER // SODIUM/POTASSIUM/CALCIUM EXCHANGER 6, MITOCHONDRIAL; CAX family cation antiporter, membrane protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAAAGAAGGGATTGCTCTTGCGTTGGTAAGTGCAGTTCACTGTATT |
| Internal bar code: | CTGATGCCAACATCCCATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2267 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTCCAGCTAGTTGCCT |
| Suggested primer 2: | ACAGATGCCAGCAGTAACCC |