Insertion junction: CLIP2.055542_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055542
Chromosome:chromosome 4
Location:680544
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAATTGTCGTCACGATTGCAAACGCCGCAGTCCAGCAACCTACGGTGA
Internal bar code:TGCTGACTGGTTTGTAGTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4896
LEAP-Seq percent confirming:83.9286
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:112

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGTCGGCGTATTACTGGG
Suggested primer 2:CACCCAGCGTGAAGTTGTTG