Insertion junction: CLIP2.055766_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055766
Chromosome:chromosome 14
Location:1139040
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614900 TSQ1 Glutaminyl-tRNA synthetase; (1 of 1) 6.1.1.18 - Glutamine--tRNA ligase / Glutaminyl-tRNA synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAAGACCGCTGCACAGCGTTGCTGGCGACTTGCTGGAAGGCTCCACCA
Internal bar code:CTTTTCCCAACCACTTGTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:13.6364
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTTAGCCATCGGGGGAGA
Suggested primer 2:AGGTACAGGGATTGCTTGCC