| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056257 |
| Chromosome: | chromosome 9 |
| Location: | 1103378 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401700 | (1 of 1) PTHR16193:SF0 - TETRATRICOPEPTIDE REPEAT PROTEIN 27 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCATTGACCATCCCGGTCCAAGTGGGCCAGTTGCAATAGTAAACACC |
| Internal bar code: | AAACTGGTGTAGAATGCGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1563 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCTGTAAACGCGGCACT |
| Suggested primer 2: | GGACCATACACGCGTACGAT |