Insertion junction: CLIP2.056268_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.056268
Chromosome:chromosome 1
Location:7760948
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g054700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGATCGAAATAGTAGTCTACTCACTTCCCTTCTAACCGGCGCGCTGGCG
Internal bar code:ACGGCAGTTATGCGCTTATATG

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGCCGAGGATGGGTATTG
Suggested primer 2:TACGCACACACACACTCTCC