| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056444 |
| Chromosome: | chromosome 9 |
| Location: | 3517635 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396401 | SUMT1:COBA,UPM1 | S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase; (1 of 1) PTHR21091//PTHR21091:SF16 - METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED // SIROHEME SYNTHASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGGTTGGAAAGCAGCCGGAGGGTTAAGAAGAGCTCGCAGAAGGCCTC |
| Internal bar code: | GGAATCCCGACTTTCAGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4649 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTCTGCGTGTGTGTTC |
| Suggested primer 2: | GGACATACGGATGAGGCCAG |