| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056754 |
| Chromosome: | chromosome 12 |
| Location: | 1905342 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511900 | RPE1 | Ribulose phosphate-3-epimerase, chloroplastic; (1 of 1) PTHR11749//PTHR11749:SF4 - RIBULOSE-5-PHOSPHATE-3-EPIMERASE // D-RIBULOSE-5-PHOSPHATE-3-EPIMERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACGACACGCGTGCCCCGCGTGTTCGAATCGATGTAAATTTTGAGTTT |
| Internal bar code: | GTGGGTTGGTTAATAGGTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4241 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGCGCGATTTTACTCA |
| Suggested primer 2: | CAAGTAGGGATCGGTACCGC |