| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057110 |
| Chromosome: | chromosome 12 |
| Location: | 4877470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523850 | MPPB,QCR1 | (1 of 1) K17732 - mitochondrial-processing peptidase subunit beta [EC:3.4.24.64] (PMPCB, MAS1); Ubiquinol:cytochrome c oxidoreductase 50 kDa core 1 subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATGTGAAAAGGAGAGCCCAAAGGCTGGGTAATCACTGGGGACCGATG |
| Internal bar code: | TATGTCAGGTCCTTAAGACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1351 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGGGTAAGCTCCATCT |
| Suggested primer 2: | GATCAGGTGGCCAAGAAGCT |