Insertion junction: CLIP2.057331_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057331
Chromosome:chromosome 2
Location:2354330
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g090750 (1 of 1) PF08574 - Protein of unknown function (DUF1762) (DUF1762) 3'UTR
Cre02.g090850 CLPB3 (1 of 1) PTHR11638//PTHR11638:SF121 - ATP-DEPENDENT CLP PROTEASE // CHAPERONE PROTEIN CLPB3, CHLOROPLASTIC; ClpB chaperone, Hsp100 family 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCCGCGCCAACGCGCCGTCGAACCCCTCCAACCCCCCCGAAAGCATG
Internal bar code:CATCAGTACTTATATATCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1120
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCAACTCGACCAGACTCA
Suggested primer 2:GCAAGCCGGCTGGATACTAT