Insertion junction: CLIP2.057405_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057405
Chromosome:chromosome 3
Location:1452911
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTGTCAATCGTTTCGGTGTTTGGCGAGAGTACCCAGACCCTTCACGGA
Internal bar code:GTTGGTTTAATAGATAAGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1231
LEAP-Seq percent confirming:89.6552
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGCAGGGTTTCGATATCG
Suggested primer 2:AAGACACACAGCTTACCGGG