| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057845 |
| Chromosome: | chromosome 16 |
| Location: | 6036271 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678100 | VPS28 | (1 of 1) K12184 - ESCRT-I complex subunit VPS28 (VPS28); Subunit of the ESCRT-I complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCATGCCCATTCCCGCCCGCGCCTGCCCACACCAAACCGCGCCATCCT |
| Internal bar code: | GAATACAAGGCATGCTCGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGCACCAAACAGAAGTG |
| Suggested primer 2: | GCACATGTACAGCGCATCAG |