| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.057970 |
| Chromosome: | chromosome 3 |
| Location: | 7442432 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205900 | DCA1 | (1 of 1) 4.1.1.50 - Adenosylmethionine decarboxylase / S-adenosyl-L-methionine decarboxylase; S-adenosylmethionine decarboxylase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCCCGAAAACCCTCCGCGCGCAAACACCTCAACTATGTCTATCAGGC |
| Internal bar code: | CATGATAACGGGATTTTACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2386 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTAGATCACCTACAGC |
| Suggested primer 2: | GAGTTCAGCAGGCGTGTAGT |