Insertion junction: CLIP2.057996_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.057996
Chromosome:chromosome 13
Location:1319400
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571200 HKR5,HIK5,HIK (1 of 1) IPR000014//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006 - PAS domain // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily; Histidine kinase response regulator of two-component system 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACTTTATTTGTGACGCCGTGCAAGAGCTCAAGCGCTCGGAATGCGCTT
Internal bar code:TGGGGCACTTGATGATGAGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1753
LEAP-Seq percent confirming:80.8511
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GACAGTTGAAGGTCCCCGAG
Suggested primer 2:GGCCCTGTACTCTGCTGTTT